Poly-epsilon-caprolactone Nanofibrous Polymers: A Simple Alternative to Restricted Access Media for Extraction of Small Molecules from Biological Matrixes

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dc.contributor.author Raabová, Hedvika
dc.contributor.author Háková, Martina
dc.contributor.author Chocholoušová Havlíková, Lucie
dc.contributor.author Erben, Jakub
dc.contributor.author Chvojka, Jiří
dc.contributor.author Solich, Petr
dc.contributor.author Švec, František
dc.contributor.author Šatínský, Dalibor
dc.date.accessioned 2020-06-17T06:49:50Z
dc.date.available 2020-06-17T06:49:50Z
dc.date.issued 2020-05-19
dc.identifier.uri https://dspace.tul.cz/handle/15240/157195
dc.identifier.uri https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.0c00544
dc.description.abstract Poly-epsilon-caprolactone nanofibrous polymer has been used as an alternative to restricted access media for extraction of protein-containing biological samples and direct transfer in the chromatographic system. Three commercial cartridges differing in length and internal diameter have been manually packed with the composite material prepared from poly-epsilon-caprolactone nanofibers coated on poly-epsilon-caprolactone microfibrous scaffold and connected to the column-switching chromatographic system. Bovine milk and human serum (25 mu L) spiked with a mixture of methyl-, ethyl-, propyl-, and butylparaben in a concentration range of 1-100 mu g mL(-1) were online extracted using the cartridge-containing fibers. Then, 5 and 20% (v/v) aqueous methanol was applied as the washing mobile phase. While the ballast protein macromolecules were quantitatively eluted from the nano/microfibrous composite sorbent, the parabens were retained. After the mobile phase was switched to a stronger one, these compounds were then eluted from the extraction sorbent, directed in the analytical column, and finally separated. An extraction efficiency of 86-101% for all parabens achieved using the optimum-sized cartridge and a repeatability of the extraction procedure of 0.06-1.95% RSD were obtained. cs
dc.format.extent 5 stran cs
dc.language.iso cs cs
dc.language.iso cs cs
dc.publisher AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
dc.relation.ispartof ANALYTICAL CHEMISTRY
dc.subject PERFORMANCE LIQUID-CHROMATOGRAPHY cs
dc.subject SOLID-PHASE EXTRACTION cs
dc.subject SAMPLE PREPARATION cs
dc.subject EFFICIENCY cs
dc.subject COLUMN cs
dc.title Poly-epsilon-caprolactone Nanofibrous Polymers: A Simple Alternative to Restricted Access Media for Extraction of Small Molecules from Biological Matrixes cs
dc.identifier.doi 10.1021/acs.analchem.0c00544
local.relation.volume 92
local.relation.issue 10
local.citation.spage 6801
local.citation.epage 6805
dc.identifier.orcid 0000-0001-9856-1604 Erben, Jakub
dc.identifier.orcid 0000-0001-8137-7878 Chvojka, Jiří


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