Experimental fortification of intestinal anastomoses with nanofibrous materials in a large animal model

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dc.contributor.author Rosendorf, Jáchym
dc.contributor.author Horáková, Jana
dc.contributor.author Klíčová, Markéta
dc.contributor.author Pálek, Richard
dc.contributor.author Červenková, Lenka
dc.contributor.author Kural, Tomáš
dc.contributor.author Hošek, Petr
dc.contributor.author Kříž, Tomáš
dc.contributor.author Tégl, Václav
dc.contributor.author Moulisová, Vladimíra
dc.contributor.author Tonar, Zbyněk
dc.contributor.author Treska, Vladislav
dc.contributor.author Lukáš, David
dc.contributor.author Liska, Václav
dc.date.accessioned 2020-02-11T08:22:35Z
dc.date.available 2020-02-11T08:22:35Z
dc.date.issued 2020
dc.identifier.uri https://dspace.tul.cz/handle/15240/154399
dc.identifier.uri https://www.nature.com/articles/s41598-020-58113-4
dc.description.abstract Anastomotic leakage is a severe complication in gastrointestinal surgery. It is often a reason for reoperation together with intestinal passage blockage due to formation of peritoneal adhesions. Different materials as local prevention of these complications have been studied, none of which are nowadays routinely used in clinical practice. Nanofabrics created proved to promote healing with their structure similar to extracellular matrix. We decided to study their impact on anastomotic healing and formation of peritoneal adhesions. We performed an experiment on 24 piglets. We constructed 3 hand sutured end-to-end anastomoses on the small intestine of each pig. We covered the anastomoses with a sheet of polycaprolactone nanomaterial in the first experimental group, with a sheet of copolymer of polylactic acid with polycaprolactone in the second one and no fortifying material was used in the Control group. The animals were sacrificed after 3 weeks of observation. Clinical, biochemical and macroscopic signs of anastomotic leakage or intestinal obstruction were monitored, the quality of the scar tissue was assessed histologically, and a newly developed scoring system was employed to evaluate the presence of adhesions. The material is easy to manipulate with. There was no mortality or major morbidity in our groups. No statistical difference was found inbetween the groups in the matter of level of peritoneal adhesions or the quality of the anastomoses. We created a new adhesion scoring system. The material appears to be safe however needs to be studied further to prove itsʹ positive effects. cs
dc.format.extent 12 stran cs
dc.language.iso cs cs
dc.publisher Nature Research
dc.relation.ispartof Scientific Reports
dc.subject Anastomotic Leak cs
dc.subject Stoma closure cs
dc.subject Rectal Neoplasms cs
dc.title Experimental fortification of intestinal anastomoses with nanofibrous materials in a large animal model cs
dc.identifier.doi 10.1038/s41598-020-58113-4
local.relation.volume 10
local.relation.issue 1
local.access open access
local.identifier.publikace 7087
dc.identifier.orcid 0000-0003-2926-0570 Horáková, Jana
dc.identifier.orcid 0000-0003-4037-6435 Lukáš, David
local.article.number 1134
local.identifier.WebofScienceResearcherID A-8450-2018 Horáková, Jana
local.identifier.WebofScienceResearcherID G-9288-2014 Lukáš, David


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